Recent applications of ATR FTIR spectroscopy and imaging to proteins
نویسندگان
چکیده
منابع مشابه
Chemical imaging of microfluidic flows using ATR-FTIR spectroscopy.
Elucidating the chemical composition of microfluidic flows is crucial in both understanding and optimising reactive processes within small-volume environments. Herein we report the implementation of a novel detection methodology based on Attenuated Total Reflection (ATR)-Fourier Transform Infra-Red (FTIR) spectroscopic imaging using an infrared focal plane array detector for microfluidic applic...
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This study is aimed at evaluating the performance of tetrakismethylene-3-3,5-di-tert-butyl-4-hydroxyphenyl propionate methane S1010, tris2,4-di-tert-butylphenyl phosphite S1680 and ascorbic acid AA antioxidants in stabilization of hydroxypropyl cellulose HPC. For this purpose, a number of films with weight percent ratios of 0.4 w/w were prepared in a 1 isopropanol solution w/v. Then, the films ...
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Polarized FTIR ATR spectroscopy was used to investigate the interaction of mitochondrial creatine kinase (Mi-CK) and intestinal alkaline phosphatase (AP) with model membrane assemblies. Mi-CK was immobilized by adsorption to the negatively charged cardiolipin (CL) leaflet of a supported CL/DPPA bilayer. H-D-exchange of the enzyme and the stability under flowthrough conditions of the protein/mem...
متن کاملRapid discrimination of maggots utilising ATR-FTIR spectroscopy.
Entomological evidence is used in forensic investigations to indicate time since death. The species and age of maggots or flies that are present at the scene can be used when estimating how much time has passed since death. Current methods that are used to identify species and developmental stage of larvae and fly samples are highly subjective, costly and often time consuming processes and requ...
متن کاملATR–FTIR spectroscopy reveals involvement of lipids and proteins of intact pea pollen grains to heat stress tolerance
With climate change, pea will be more frequently subjected to heat stress in semi-arid regions like Saskatchewan during flowering. The pollen germination percentage of two pea cultivars was reduced by heat stress (36°C) with an important decrease in cultivar 'CDC Golden' compared to 'CDC Sage.' Lipids, protein and other pollen coat compositions of whole intact pollen grains of both pea cultivar...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
سال: 2013
ISSN: 1570-9639
DOI: 10.1016/j.bbapap.2013.07.015